In vitro propagation of the peach rootstock: the effect of different carbon sources and types of sealing material on rooting
Fotopoulos, S.(Pomology Institute, Naoussa (Greece)) | Sotiropoulos, T.E.(Pomology Institute, Naoussa (Greece))E-mail:[email protected]
The objectives of the present research were to study the effect of sucrose and glucose as carbon sources at various concentrations as well as the effect of different sealing materials (aluminium foil, cotton, rubber, parafilm) on the rooting response of the PR 204/84 peach (Prunus persica x P. amygdalus) shoots cultured in vitro. The cultures of the PR 204/84 peach rootstock produced the highest rooting percentage, mean root number, mean root length, fresh, and dry mass of roots when grown on media containing 88 mM sucrose or 88 mM glucose. Parafilm, rubber and aluminum foil as sealing materials were not significantly different in terms of rooting percentage, fresh, and dry mass of roots after 24 d in culture. The use of cotton as sealing material induced lower root number per shoot, length of roots, fresh, and dry mass of roots than the other treatments.
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