Response of wheat crosses involving salt tolerant genotypes to anther culture
2002
Sehrawat, A.R. | Uppal, S | Singh, K.P. (C.C.S.H.A.U., Hisar (India). Department of Genetics)
Anthers of seven wheat (Triticum aestivum L) genotypes and their eight F1 hybrids involving Kharchia375 and Kharchia65, salt tolerant cultivars, as fremale parents were cultured on four modified media, both solid and liquid. The per cent anthers forming calli varied between 0 and 9.69 depending upon the genotype, cross and culture medium. Calli yielded both green (upto 5.6%) and albino plantlets (8.5 6o48.9%) on transfer to solidified agar 198-2 regeneration medium. Crosses differed significantly and the best cross with respect to callusing and green plantlet regeneration was Kharchia375 x HD2009. In general responses of different crosses were significantly better in liquid media and the most effective medium overall was P2 followed by P4, BAC1 and Heh. Higher green plantlet regeneration abilities from anthers of F1 hybrids as compared to the parents suggest their use to increase green plant yield. Plantlets thus produced from crosses after diploidization were transferred to pots. Ninety-seven fertile spikes obtained from crosses showed a wide variation for spike length, spikelet number per spike and grain number per spike. The study may be helpful for the development of salt tolerant doubled haploid lines of wheat.
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