Genetic variation in gamma ray induced mutants in blackgram as revealed by RAPD and ISSR markers
2002
Souframanien, J | Pawar, S.E. | Rucha, A.G. (Bhabha Atomic Reseach Centre, Mumbai (India). Nuclear Agriculture and Biotechnology Division)
Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in 12 gamma ray induced morphological mutants of blackgram [Vigna mungo (L.) Hepper]. OF the 35 random and eight ISSR primers used, 15 random and five ISSR primers detected polymorphism among the mutants. Total number of bands varied from 2 to 9 for RAPD and from 6 to 10 for ISSR. The number of polymorphic bands varied from 1 to 3 for RAPD and from 1 to 6 for ISSR. Percentage of polymorphism ranged from 12.5 to 50.0 for RAPD and 12.5 to 44.4 for ISSR. Significant DNA polymorphism among the mutants were observed using RAPD (25.8%) and ISSR (33.3%) marker. An young leaf chlorine mutant and a smooth pod mutant showed more DNA polymorphism as compared to the parent. DNA polymorphism data revealed by RAPD and ISSR could facilitate selection of mutants to be involved in cross breeding and genome mapping.
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