Ecological characteristics of Escherichia coli infection in hydroponics and development of methods of control
2001
Shirakawa, T. (National Research Inst. of Vegetables, Ornamental Plants and Tea, Ano, Mie (Japan)) | Abiko, K.
Ecological characteristics and methods of prevention of E. coli infection in hydroponic culture were studied using rifampicin and nalidixic acid-resistant mutant of E. coli. The population of E. coli that were inoculated to the hydroponic culture fluid of lettuce and Japanese hornwort decreased slowly and E. coli were not detected by the dilution plating technique in culture fluid 7 days after the inoculation. However, E. coli survived in the roots of the crops and in the culture fluid in the urethane medium 14 days after inoculation. Also, inoculated E. coli were detected by the enrichment culture technique from the culture fluid 14 days after inoculation. E. coli that infested on the apparatus and materials used for hydroponics contaminated with E. coli could be disinfected by heat treatment or NaClO treatment. Lettus seeds inoculated with E. coli were disinfedted by hot air treatment at 80 deg C for 3 days. Treatment of the hydroponic culture fluid with NaClO was not effective in the elimination of E. coli and E. coli were detected from the culture fluid 14 days after NaClO treatment by the enrichment technique.
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