Development and quality analysis of a selective medium for isolation of Escherichia coli from agricultural ecosystems
2001
Shirakawa, T. (National Research Inst. of Vegetables, Ornamental Plants and Tea, Ano, Mie (Japan)) | Abiko, K.
A selective medium (NIVOT-EC) was developed for quantitative isolation and detection of Escherichia coli from soil and plant materials. NIVOT-EC consisted mainly of mineral salts. On NIVOT-EC, E. coli formed characteristic blue colonies, when incubated at 36 deg C. Plating efficiency of E. coli NIVOT-EC was higher than that on 7 tested selective media developed for the detection of E. coli. More than 99.9% of the rhizosphere soil bacteria were unable to grow on NIVOT-EC. Among 56 bacterial strains (except for E. coli) tested, only 7 strains in 4 genera could grow on NIVOT-EC, when incubated at 36 deg C. On the other hand, 21 - 47 strains in 8 - 9 genera could grow 7 existing selective media tested. Based on comparative tests with 8 known selective media, NIVOT-EC was found to be the most efficient selective medium for quantitative isolation of E. coli from agricultural ecosystems.
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