Nuclear transfer using cumulus-oocytes complexes from individual ovaries of slaughtered cows
2001
Hasegawa, K. (Shimane-ken. Animal Husbandry Experiment Station, Izumo (Japan)) | Akagi, S. | Takahashi, S. | Izaike, Y. | Okazaki, T. | Abe, S.
We studied the efficiency of in vitro development of somatic cell-nuclear transferred embryos reconstructed by fusing a cumulus cell and oocyte obtained from cumulus-oocytes complexes (COCs) from ovaries of slaughtered cows. The cumulus cells were treated in two groups (Group I and II) before nuclear transfer as follows. Group I : cumulus cells derived from COCs were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and followed by three passages. Furthermore, the cells were cultured for 5 days in DMEM supplemented with 0.5% FBS. Group Hicumulus cells were derived from COCs after in vitro maturation for 20hr in TCM-199 supplemented with 10% FBS. The fusion rate in Group I was higher than Group II(88.2% vs. 67.1%, P<0.01). The cleavage rate of fused eggs (86.7% vs. 75.4%, P<0.05) and the developmental rate to the blastcysts stage (43.3% vs. 29.4%, P<0.01) were similar. The average number of blastcysts per an ovary(mean +- S.D.) did not differ significantly between Group I and II (2.6 +- 2.2 and 1.7 +- 2.0). The number of blastcysts developed from an ovary dispersed in each ovaries and ranged from 0 to 10. Our results demonstrated that donor cumulus cells applied either these pre-treatments develop to transferable embryos by somatic nuclear transfer when COCs from individual ovaries of slaughtered cows use. These data suggested that this method can be applied to OPU technique to obtain oocytes and cumulus cells for somatic nuclear transfer.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Agriculture, Forestry and Fisheries Research Information Technology Center