Character of tobacco mosaic virus isolated from tomato [Lycopersicon esculentum] plants showing necrotic disease
2001
Fukuda, A. (Fukui-ken. Agricultural Experiment Station (Japan)) | Komano, M. | Honda, N.
We examined the host range, the gene analysis of coat protein and the control of Tobacco mosaic virus F28 that occured necrotic fruits in gMidih-type Tomato gKoshi-no-Rubyh. TMV-F28 and TMV-OM coat protein gene have nucleotide and amino acid homologies of 98.5 and 98.8. Out of 3 cultivar 40 plant species belonging 14 families, which were inoculated with sap of TMV-F28, 3 cultivar 21 species belonging to 7 family were infected. Host range and their symptoms were similar to TMV-OM. For rapid and convenient detection of TMV-F28,we examined the detectable condition of the modified indirect ELISA(T-ELIS4). TMV was specifically detected by using antigen diluted l:10000, antiserum diluted l:20,000 in the sap of tomato plant infected with ToMV, and anti-rabbit IgG alkaline phoshatase conjugate diluted l:4000. Using an Attenuated tomato mosaic virus ToMV-L sub (11) A, we investigated cross protection against TMV-F28. Pre-inoculation of Tomato plants with ToMV-L sub (11) A didnft protected from infection with TMV-F28, since it induced mosaic leaf symptoms and necrotic fruits. TMV-F28 inoculated 25 resistant cultevars, it caused systemic invasion followed by mosaic or chlorosis on cultevars containing resistance gene Tm-1. On cultevars with the resistance gene Tm-2 or Tm-2 sub (4), the virus induced necrotic local lesions on the inoculated leaves, necrosis on the upper leaves and stunt. But it was not observed symptoms by the virus on tomato cultivar gOrange-carolh with resistant geneiTm-2/+j.
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