Gender-related differences in mouse hepatic ethanol metabolism
2002
"Kishimoto, R. (Kobe Gakuin Univ. (Japan). Faculty of Nutrition) | Ogishi, Y. | Ueda, M. | Matsusaki, M. | Amako, K. | Goda, K. | Park, S.S. "
"The effect of an acute oral load of 2 g ethanol/kg body weight was studied in a group of male and female 10-wk-old C3H/HeNCrj (C3H/He) mice to investigate gender change throughout differences of the hepatic ethanol metabolism of mice. The following parameters were measured in the serum from 0h to 3h after the start of the experiment: ethanol, acetaldehyde, and acetate. Their concentrations in the serum in female mice tended to show lower levels than in male mice. In female mice, the concentration of ethanol at 1h and the concentration of acetate at 1h, 2h, and 3h after ethanol administration showed significantly lower levels than in male mice. Ten-week-old male and female C3H/He mice were subcutaneously injected 50 micro g/kg body weight beta-estradiol and 1.45mmol/kg body weight testosterone propionate (testosterone) in olive oil, respectively, and changes in the activity of enzymes related to the hepatic ethanol metabolism of mice were examined at 24h after the administration of sex hormones. The activity of the cytosolic alcohol dehydrogenase (ADH) and microsomal anlline hydroxylase (ANH) and the low Km, high Km and total aldehyde dehydrogenase (AlDH) activities in the mitochondrial, the cytosolic, and the microsomal fraction of the liver were higher. Moreover, the denslty of the band of CYP2El in the microsome in female mice was stronger than in male mlce, and in the microsomal fraction of the liver, the total content of cytochrome P-450 (CYP) and ethoxyresorufin omicron-dealkylase (EROD) activity in male mice showed significantly higher values than in female mice. The density of the band of CYP2El and the three activities of AlDH in the hepatic mitochondorial fraction of male mice increased significantly under treatment with beta-estradiol."
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