Virus content test without using antiserum for canine adenovirus and canine parainfluenza virus in canine live combined vaccines
2003
Senda, M. (Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.) | Kobayashi, R. | Gamoh, K. | Itoh, O.
Most virus content tests for canine adenovirus and canine parainfluenza virus in canine live vaccines are performed using MDCK cells. In the case of combined vaccines, vaccine viruses other than the target virus are neutralized before titration. However, the use of polyvalent vaccines with high virus titers has increased and the preparation of hyperimmune serum sufficient to neutralise each vaccine virus in such vaccines has proved difficult. Virus content tests using indicators other than CPE do not need antiserum. For example, canine parainfluenza virus shows hemagglutination in inflected cells or culture fluid. In this study, monoclonal antibodies (MAbs) to canine adenovirus were prepared and an indirect immunoperoxidase method using the MAb was established with infected cells. Combining this procedure with the canine parainfluenza hemagglutination test enables the simultaneous titration of canine adenovirus and canine parainfluenza virus in combined live vaccines without the need for neutralization. In this combined assay, culture fluid is used for the canine parainfluenza virus titration and infected cells are used for the canine adenovirus titration.
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