Effect of wood matrix moisture content on the degradation rate and microbial composition in the household garbage automatic decomposer-extinguisher (GADE) machine
2003
Horisawa, S. (Akita Univ. (Japan)) | Morishita, T. | Sakuma, Y. | Doi, S. | Terazawa, M.
Abstract To determine the optimum wood matrix moisture content for the GADE machine, model waste degradation experiments were conducted. The controlled wood matrix moisture content was adjusted to 30%, 40%, 50%, and 60%, respectively. Rate of weight loss, temperature of the matrix, and ATP content in each matrix were evaluated for 15 days. The weight loss rates were similar at moisture content levels of 40%-60%, even though high temperatures and ATP levels were observed at moisture content levels of 40% and 50%. The matrix microbial communities were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) using a nucleotide sequence of bacterial 1 6S rDNA. The microbial community structures were stable from the 5th to the 15th day in each moisture content container and were also similar among the moisture content levels except for 30% level. The degradation rates were appeared to differ under different environmental conditions (moisture content and temperature) even when the microbial communities were similar. Our results indicate that the optimum moisture content is 40%-50% in the household GADE machine.
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