Characterization of the Nucleotide Sequence of a Polyubiquitin Gene (PUBC1) from Arabian Camel, Camelus dromedarius
Abdulaziz Ali A. Al-Khedhairy(King Saud University, Riyadh, Saudi Arabia)E-mail:[email protected]
Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin.
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