Comparison studies among the methods used in isolating the GSH frombaker yeast
2005
Salleh, M.R.B.M. | Farouk, A.E. | Jamal, P. | Hashim, Y.Z.H.B. | Salleh | R.B.,International Islamic Univ. Malaysia, Kuala Lumpur (Malaysia).Dept. of Biotechnology Engineering
The purpose of this study was to make a comparison among the methodsused in isolating the GSH from baker yeast. This was done by comparing theoptimum values of operating parameters such as the yeast concentration,processing temperature and the isolation time of each of the isolation methodsused (Autolysis, Ultrasonic and Homogenizer). Among the three methods,Autolysis method produced the highest GSH concentration of 49.263 mu mol mL-1 (15.14 g L-1) followed by Homogenizer method (18.33 mu mol mL-1 or 5.63 gL-1) which is slightly higher than that of the Ultrasonic method (18.13 mu molmL-1 or 5.57 g L-1). The different occurred probably due to the different ofthe isolation time applied to the three methods. The same duration timecouldn't be used because it will increase the temperature of the sample higherthat the optimum temperature of some of the methods. Unlike Ultrasonic andHomogenizer methods, the temperature for Autolysis method could bemaintained within I h while for Homogenizer and Ultrasonic methods thetemperature could only be maintained within 30 and 15 sec, respectively. Thestep down arrangement from best to worst for lab scale production wasAutolysis greater than Homogenizer greater than Ultrasonic method. Autolysiswas the best but the isolation time is longer compared to the other so that inlarge scale production, Homogenizer seems to be the best method due to theeconomical perspective. Ultrasonic is also can be considered as an excellentmethod but expensive compared to others.
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