Map-based cloning of Ps1, a gene for pollen abortion in rice
2003
Lin, S.Y. | Takashi, T. | Sasaki, T. | Yano, M.
Abortions of male and female gametes are frequently observed in F2 and advanced progenies from distant crosses of rice. We detected a gene, Ps1, for pollen abortion in BC4F4 backcross progenies derived from a cross between japonica variety Nipponbare and indica variety Kasalath. The Ps1 gene was mapped onto chromosome 1 by a linkage analysis with RFLP markers. Further progeny analysis using RFLP markers revealed that male gametes carrying the Nipponbare allele at the Ps1 locus were aborted in plants heterozygous at the Ps1 locus. To isolate the Ps1 gene, we constructed a high-resolution genetic linkage map of the Ps1 region, the Ps1 gene was mapped into the interval between RFLP markers G270 and E60083. Four P1-derived artificial chromosome (PAC) clones were selected from the genomic library of Nipponbare by two sequence-tagged sites of G270 and E60083. By genetic mapping of subclones derived from a PAC clone containing the Ps1 locus, a 20-kb region was defined as a candidate genomic region for Ps1.
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