Transforming indica rice cultivars grown in Vietnam using Agrobacterium tumefaciens or particle bombardment
2003
Bong, B.B. | Cuc Hoa, T.T. | Hodges, T.K. | Christou, P.
Genetic transformation mediated by Agrobacterium tumefaciens was performed in four indica cultivars grown in Vietnam and in two japonica cultivars. Embryogenic calli derived from mature seeds were inoculated with A. tumefaciens strain LBA4404, in which the T-DNA contained CaMV 35S-lox-hyg-lox-gusA or Ubi-Cre-CaMV 35S-hyg or Ubi-Cre-CaMV 35S-barB. Transformation efficiency, which is recorded as the number of independent transgenic plants showing Southern blot+ per number of calli inoculated, was 2.2-10.1% for indica cultivars and 10.4-25.8% for japonica cultivars. Southern analyses of T0 transgenic plants and T1 and T2 progenies revealed that most of the transgenes were integrated in the genome as a single locus and segregated following Mendelian inheritance. By using particle bombardment, we cotransformed the two insecticidal genes crylAc and GNA in scented indica variety KDM105. The transgenic T0 plants expressing both genes were identified by western blot analysis. In the T4 generation, a few lines showed resistance to brown planthopper in the seed-box test.
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