Study of suitable techniques in tissue-culture propagation of Mali-Ong banana
1999
Nongkran Kanchanaprasert | Tassanee Siriwan (Rajabhat Institute Pibulsongkram, Phitsanulok (Thailand))
Study of suitable techniques in tissue-culture propagation of Mali-Ong banana by using shoot tips, shoot tips with several leaf sheaths and cutting shoot tips in to 4 pieces from 20, 50 and 70 cm in length of suckers and Murashige and Skoog's (1962) medium, with was supplemented with growth regulators in 3 combinations. The results revealed that growth and development of explants for shoot tips cultures and shoot tips with several leaf sheath cultures on medium I (MS + BA 5 mg/1.) were same as medium II (MS + Kn 2.5 mg/l + BA 2.5 mg/l) callus was found, that unsuitable for propagation of Mali-Ong banana, and were the same as for 20, 50 and 70 cm in length of suckers. After subcultured on medium I and medium II, at the end of 7 months the everage of number of plantlets by using shoot tips, shoot tips with several leaf sheaths and cutting shoot tips in to 4 pieces were 147, 154 and 393 plantlets/1 sucker, which was significantly different at .01 level, and by using medium I and II were not different.
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