Simplified rapid screening method for aflatoxin in maize improvement of minicolumn method
1992
Suparut Kositcharoenkul | Prisnar Siriacha | Prawat Tonboon-ek | Kanjana Bhudhasamai | Tanaka, Toshitsugu | Arai, Katsusuke (Department of Agriculture, Bangkok (Thailand). Plant Pathology and Microbiology Div.)
Although the minicolumn method is one of the simple aflatoxin detection methods widely used for inspection in food and feed staffs, it has many steps and need a number of chemicals and laboratory equipments in current method. To improve such defects and time consuming modification of minicolumn method was attempted. The composition of chemicals in the modified minicolumn method used only 2 chemicals, Florisil and silica gel in the column, and applied the capillary action to develop the sample solution by simply dipping the column in the extract through filter paper. In addition, extraction method using ultrasonicator, shaker and waring blender were investigested. The ultrasonic extraction method using chloroform and methanol (97:3) for 10 minutes showed satisfactory result. Aflatoxin contamination level as low as 10 ppb could be detected under ultraviolet light (365 nm) observing fluorescent intensity as compared with the standard aflatoxins, and time required only 25 minutes. Percentage of collected result compared with the CB method of AOAC was 75 percent.
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