Molecular analysis of gene for phospholipase C originating from Nicotiana tabacum L. (NtPLC1)
2004
Kondic-Spika, A.
The gene for phospholipase C belongs to a group of stress-induced genes. The assess its role in plant tolerance to stress, a detailed analysis was conducted of NtPLC1 cDNA clone, isolated in PMB Laboratory of ICGEB Center, New Delhi, India. A computer analysis has shown that the NtPLC1 cDNA clone is 2104 bp long, with the open reading frame (ORF) of 1775 bp. Intron presence has not been confirmed in the genomic organization of the NtPLC1 gene. The ORF encodes a 586 amino acid protein, which is very similar to other plant PI-PLC proteins, regarding its characteristics and structural organization. Agrobacterium tumefaciens - mediated transformations of tobacco with the NtPLC1 gene have been done too. PCR has shown that the gene was successfully introduced in sense orientation in 10 regenerated tobacco plants, while it was introgressed in antisense orientation in 22 plants. Additionally, 5 plants were regenerated in each of the two control groups: the plants transformed with the vector alone and the wild type. There were no differences at phenotype level among the groups of regenerants. The NtPLC1-sense and antisense transgenic tobacco plants made in this study are expected to exhibit differential reactions to stress conditions. If this proves true, a subsequent study would cast light on the role of phospholipase C in signal transduction, i.e. in plant tolerance to stress.
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