Polypeptide markers for low temperature stress during seed germination in sunflower
2006
Kumar, A.(University of Delhi (India). Dept. of Botany) | Bhatla, S.C.(University of Delhi (India). Dept. of Botany) E-mail:[email protected]
Sunflower seeds behaved as chilling and freezing sensitive and also exhibited acclimation under low seed moisture content (less than 1%). At high seed moisture content (approx. 22%) they tolerated chilling stress but failed to acclimate under freezing temperatures. Pre-imbibitional chilling (5 deg C) or freezing (-5 or -10 deg C) stress significantly enhanced total soluble protein (TSP) concentration. Chilling treatment after imbibition (in contrast to pre-imbibition) enhanced germination and this was accompanied by increase in 30, 24 and 21.9 kDa TSPs contents (3 d after germination). Freezing at -5 and -10 deg C suppressed seed germination and increased the concentration of 78 and 56.2 kDa wall bound proteins. Chilling acclimation decreased 35.4, 33.9, 29.5, 23.4 and 21.4 kDa TSPs.
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