Detection of phytoplasma ESFY in apricot trees using phloem and petioles
2005
Necas, T.(Mendelova Zemedelska a Lesnicka Univ., Lednice (Czech Republic). Ustav Ovocnictvi) E-mail:[email protected] | Krska, B.(Mendelova Zemedelska a Lesnicka Univ., Lednice (Czech Republic). Ustav Ovocnictvi)
The aim of this study was to determine the best period for tissue extraction and the best technique for European stone fruit yellows phytoplasma (ESFY) detection, as well as the possibility to isolate DNA in order to detect ESFY in the leaf-stalks of randomly chosen symptomatic and asymptomatic apricot trees. Results of the amplification of DNA extracted from leaf-stalk and phloem sampled from 2 year old woody shoots during the years 2003 and 2004 were statistically analysed and compared. Visible disease symptoms were simultaneously evaluated and compared with the results of molecular detection. DNA isolation from leaf-stalks can be considered as less significant and reliable than isolation from phloem sampled from 2 year old woody shoots.
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