Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD
2006
Padmesh, P.(Tropical Botanic Garden and Research Inst., Thiruvananthapuram (India)) E-mail:[email protected] | Reji, J.V.(Tropical Botanic Garden and Research Inst., Thiruvananthapuram (India)) | Jinish Dhar, M.(KSR Coll. of Arts and Science, Salem (India)) | Seeni, S.(Tropical Botanic Garden and Research Inst., Thiruvananthapuram (India))
Genetic diversity was estimated in 13 accessions of Mucuna pruriens comprising varieties pruriens and utilis collected using 15 RAPD primers. Similarity index value of 0.68 indicated high degree of genetic variability. Analysis of various genetic diversity indices like total heterozygosity, Nei's gene diversity, percentage of polymorphic loci, expected and observed number of alleles and Shannon index strongly suggested that variety pruriens was genetically more diverse than variety utilis. Chemical analysis with respect to 3,4-dihydroxy-L-phenylalanine (L-DOPA) content showed uniform distribution. Cluster analysis showed grouping of accessions into two major clusters and tendency of accessions of variety pruriens to group according to their geographical locations. Bootstrap analysis confirmed the robustness of the phenogram. The putative hybrid MMP6 with relatively low similarity value index and low L-DOPA content was promising as food or fodder.
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