The simultaneous detection method of 18 species of food-born pathogenic bacteria by multiplex PCR
2003
Taguri, T. (Nagasaki-ken. Inst. of Public Health and Environmental Sciences (Japan)) | Noguchi, H. | Hirayama, H.
The Simultaneous detection method of 18 species of Food-born Pathogenic Bacteria was developed by multiplex PCR. The 18 species of Food-born Pathogenic Bacteria examined were Enterohemorrhagic Escherichia coli,Enterotoxigenic Escherichia coli,Enteroinvasive Escherichia coli,Salmonella typhi. Salmonella spp.,Shigella spp., Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus,Clostridium perfringens,Campylobacter jejuni,Campylobacter coli,Listeria monocytogenes,Yersinia enterocolitica.Yersinia pseudotuberculosis,Bacillus cereus,Staphylococcus aureus and Clostridium botulinum. At first,We picked up some used primers in reference, which were authorized by specificity and sensitivity, and then selected the best sets out by gradient thermal cycling condition. Secondly, We grouped them into multipurpose applications (ex. E.coli group,salmonella and shigella group,genus Vibrio group and so on) and decided the optimum concentration ratio on them by examined competitive interaction.As saving the time, the labor and the cost for which programed any primers and proved their specificity and sensitivity, we were able to present the new method which was rapid, convinient and comprehensible after all.
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