Silkworm assay for Bacillus thuringiensis formulations using the diet incorporation method, 4: Evaluation based on mortality at one and two dose levels
2006
Asano, S.(Kubota Co. Ltd., Ryugasaki, Ibaraki (Japan). Advanced Technology Lab.)
A silkworm assay using the 3rd instar stage of silkworm, Bombyx mori and a diet incorporation method has been utilized for the quality control of Bacillus thuringiensis (Bt) products in Japan since 1973. The potency of test sample relative to a standard was calculated based on lethal activity (LC50). The LC50 values were usually estimated by probit analysis on the mortality at the levels of 5-6 doses. In the present study the author tried simpler silkworm assays based on the lethal activity of Bt products against the 2nd instar of silkworms using 1-2 doses. It was shown that the potency of the sample relative to the standard based on assays with 1-2 concentrations is similar to that based on assays with six concentrations.
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