Agrobacterium-mediated genetic transformation of Dianthus hybrid 'Telstar Scarlet' by using regenerable callus
2006
Fukai, S.(Kagawa Univ., Miki (Japan). Faculty of Agriculture) | Nontaswatsri, C. | Uchiyama, K.
Regenerable callus culture of Dianthus hybrid 'Telstar Scarlet' was successfully applied to Agrobacterium-mediated genetic transformation. Five days coculture with 5 mM glucose and 50 mM acetosyringone was suitable for Agrobacterium-infection. Transformed shoots were obtained from inoculated calluses selected on MS medium containing 1.0 mg/1 thidiazuron, 0.1 mg/1 a -naphthalene acetic acid, 20 g/1 sucrose, 2 g/1 gellan gum and 20-80 mg/1 G-4 18 within 20 weeks from inoculation. Transformation efficiency based on the number of calluses that produced transformed plants, and based on the total number of transformed shoots were 6 % and 27% respectively. About 10% of transformants were tetraploid. All diploid transformants grew, flowered normally and produced seeds when crossed with other Dianthus species.
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