In Vitro Maturation of Tiger Oocytes : A Case Report
Lee, H.S.;Yin, X.J.;Lee, Y.H.;Min, W.K.;Kong, I.K.(Sunchon National University, Sunchon, Republic of Korea)E-mail:[email protected] | Kim, T.S.;Choi, J.W.;Yoon, B.C.;Kim, J.I.(Uchizoo, Gwangju, Republic of Korea)
The purpose of this study was to determine the possibility of in vitro maturation of tiger oocytes. Immature oocytes were recovered from a pair of ovaries. A total of 78 oocytes was collected, of which forty three were classified as good oocytes with compact cumulus cells and uniform cytoplasm. Forty three COCs were in vitro matured at 39℃, 5% CO₂ in air atmosphere for 48 h in a IVM medium (TCM-199 supplement with 10% FBS, 0.6 mM cysteine, 0.2 mM pyruvic acid and 10 IU/mL HMG). Experiment Ⅰ: the morphologic evaluation was conducted by measuring the diameter of oocytes with or without ZP, the thickness of ZP and the diameter of cytoplasm by microeyepiece at the same magnification (×100).
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