Effects of culture vessel on in vitro morphogenesis in shoot formation of Solanum melongena L. and S. integrifolium Poir
2006
Takamura, T.(Kagawa Univ., Miki (Japan). Faculty of Agriculture) | Sugimura, T. | Tanaka, M.
The effects of peptone and culture vessel on in vitro morphogenesis in shoot formation of eggplant (Solanum melongena L.) and S. integrifolium Poir were examined. Peptone had no effect on reducing hyperhydric shoots. On the other hand, culture vessels with gas-permeability by membrane filter reduced the percentage of hyperhydric shoots as compared to sealed vessels. After transplanting to soil, the survival rate of shoots obtained from the gas-permeable vessels was higher than those from the sealed vessels. Abnormally shaped stomata as compared to those formed in vivo were observed in leaf blades of hyperhydric shoots by using scanning electron microscope. Many leaf blades of non-hyperhydric shoots in sealed vessels also had abnormally shaped stomata.
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