Isolation and Characterization of Brain-Derived Neurotrophic Factor Gene from Flounder (Paralichthys olivaceus)
2005
Lee, J.H. (Pukyong National University, Busan, Republic of Korea) | Choi, T.J. (Pukyong National University, Busan, Republic of Korea) | Nam, S.W. (Dong-Eui University, Busan, Republic of Korea) | Kim, Y.T. (Pukyong National University, Busan, Republic of Korea), E-mail: [email protected]
Brain-derived neurotrophic factor (BDNF) is a small secretory protein and a member of the nerve growth factor (NGF) gene family. We cloned the flounder BDNF gene from a flounder brain cDNA library. The nucleotide sequence of the cloned gene showed an open reading frame (ORF) consisting of 810 bp, corresponding to 269 amino acid residues. The tissue distribution of flounder BDNF was determined by reverse transcription-polymerase chain reaction (RT-PCR) in brain, embryo, and muscle tissues.
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