Control of canine respiratory and diarrheal disease using egg yolk antibodies I. Induction of antibody in hens immunized with combined antigens of Bordetella bronchiseptica, parvovirus and canine distempervirus
Lee, H.S.;Kim, J.M.;Woo, S.R.;Jung, B.Y.;Cho, Y.S.;Tark, D.S.;Lim, S.K.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)E-mail:leehsoo@nvrqs.go.kr | Yoo, H.S.(Seoul National University, Seoul, Republic of Korea) | Yoon, Y.D.;Oh, J.S.(Green Cross Veterinary Science Institute, Yongin, Republic of Korea) | Huh, W.;Mun, Y.S.(Dae Sung Microbiology Laboratory, Uiwang, Republic of Korea)
This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280~1:10,240 in sera and 1:160~1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80~1:320 and 1:64~1:256, respectively. Virus neutralization titers against canine distemper was 1:8~1:64 in sera and egg yolk.
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