Rapid identification of bacterial pathogens related with bovine respiratory diseases by using PCR
Jung, B.Y.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)E-mail:jungby@nvrqs.go.kr
Haemophilus somnus, Mycoplasma bovis and Pasteurella multocida were responsible for respiratory diseases in bovine. Methods for identifying these bacteria had poor sensitivity and specificity. In this paper, PCR assays were applied for rapid identification of H. somnus, M. bovis, P. multocida B:2 and P. multocida capsular types. The specific PCR products were amplified from H. somnus, but not from other bacteria. Ten-fold diluted H. somnus were mixed with P. multocida and then the mixed cultures were inoculated on agar plates.
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