Comparison of tissue tropism of Newcastle disease vaccine viruses by Immunohistochemistry techniques
Kim, M.J.;Kwon, Y.K.;Seong, H.W.(National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kang, S.Y.;Mo, I.P.(Chungbuk National University, Cheongju, Republic of Korea)E-mail:moip@cbu.ac.kr
Mean death time of inoculated embryonated egg is one of the methods to determine the virulence of the Newcastle disease viruses (NDV). Evaluation of tissue tropism of NDV in the host has been proposed as an another way to determine the pathogenicity of NDV based on the principal site of viral replication. To evaluate the tissue tropism among NDV, an immunohistochemistry(IHC) technique using monoclonal antibody was applied in one-day-old SPF chickens inoculated with different ND vaccine strains such as Ulster 2C, VG/GA and B1 viruses by eye drop instillation.
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