In vitro Conservation of Coleus forskohlii- an Endangered Medicinal Plant
2005
Rajasekharan, P.E. (Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore, India), E-mail: [email protected] | Ambika, S.R. (Bangalore University, Jnanabharathi, Bangalore, India) | Ganeshan, S. (Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore, India)
Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with 0.54 μM naphthalene acetic acid (NAA) and 8.87 μM benzyladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum.
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