A Protocol for High Frequency Plant Conversion from Somatic Embryos of Peanut (Arachis hypogaea L. cv. DRG-12)
2005
Rani, A. Roja (University of Hyderabad, Andhra Pradesh, India) | Padmaja, G. (University of Hyderabad, Andhra Pradesh, India), E-mail: [email protected]
A protocol was developed for somatic embryogenesis with 100% induction rate from immature zygotic embryo axes of peanut (Arachis hypogaea L. cv. DRG-12) cultured on MS medium containing 18.09 μM 2,4-D. The frequency of somatic embryogenesis (31.7%) as well as the number of somatic embryos induced per explant (6.6) decreased when the concentration of 2,4-D was increased to 72.4 μM. Morphologically abnormal somatic embryos were observed at a frequency of 43.3% on MS medium containing 72.4 μM 2,4-D.
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