Effective Multiplication of Somatic Embryos Using Suspension Culture and Regeneration in Soybean
2004
Kim, Y.J. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea) | Park, T.I. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea) | Kim, H.S. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea) | Suh, S.K. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea) | Kim, H.S. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea) | Yun, S.J. (Honam Agricultural Research Institute, National Institute of Crop Science, RDA, Iksan, Republic of Korea)
The use of liquid-medium-based procedure relative to the solid media led to a 4.5-fold increase in the number of cotyledon-stage embryos. The most efficient system for multiplication and regeneration of somatic embryos was CP6 procedure with the media MSD40/MSD20/MSM6AC/FNL0S3S3GM. However, the rate of regeneration was lower. About 71％ of the embryos with dicotyledon were continued to develop the roots after desiccation treatment and 92％ of the germinated embryos produced shoots in 10 days.
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