The Transformation of a Barley Cultivar, "Saechalssalbori" using Particle Bombardment
2004
Choi, C.H. (Dankook University, Cheonan, Republic of Korea) | Yun, C.H. (National Institute of Agricultural Biotechnology, Suwon, Republic of Korea), E-mail: [email protected] | Hwang, C.H. (Dankook University, Cheonan, Republic of Korea)
Although barley is the fourth most important cereal crop in the world next to wheat, maize, and rice, efficient transformation method has not been established using Korean domestic barley. In order to find several parameters involved in particle bombardment, Bobwhite, a wheat cultivar showing a high efficiency of tranformation was examined in parallel with to Saechalssalbori, a domestic barley. The different sets of transformation with varying amounts of DNA and gold particle were performed using immature embryos as target explants.
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