Identification of Potential Corynebacterium ammoniagenes Purine Gene Regulators Using the pur-lacZ Reporter in Escherichia coli
2004
Han, R.N. (Yonsei University, Seoul, Republic of Korea) | Chung, S.O. (CJ Co., Ichon, Republic of Korea) | Kim, S.K. (Konkuk University, Seoul, Republic of Korea) | Cho, I.H. (Yonsei University, Seoul, Republic of Korea) | Choi, K.Y. (Yonsei University, Seoul, Republic of Korea), E-mail: [email protected] | Han, J.K. (CJ Co., Ichon, Republic of Korea) | Lee, J.H. (CJ Co., Ichon, Republic of Korea)
This study has developed Corynebacterium ammoniagenes (c. ammoniagenes) purine gene transcriptional reporters (purF-lacZ and purE-lacZ) that function in Escherichia coli (E. coli) DH5a. After transformation of a C. ammoniagenes gDNA library into E. coli cells harboring either purF-lacZ or purE-lacZ, C. ammoniagenes clones were obtained that repress purF-lacZ and purE-lacZ gene expression. The potential purE and purF regulatory genes are homologous to the genes encoding transcription regulators, the regulatory subunit of RNA polymerase, and genes for purine nucleotide biosynthesis of various bacteria.
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