Development of the immunochromatographic strip for the rapid detection of Listeria spp.
2005
Jung, B.Y. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea), E-mail: jungby@nvrqs.go.kr | Jung, S.C. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea) | Kim, J.M. (National Veterinary Research and Quarantine Service, MAF, Anyang, Republic of Korea)
We developed the one-step strip based on an immunochromatographic (IC) assay for the rapid detection of Listeria spp. Genus-specific monoclonal antibody to flagella of L. monocytogenes was conjugated with 40 nm colloidal gold particles which were prepared in our laboratory. The specificity of the IC strip was tested with pure cultured bacteria. All strains of the genus of Listeria spp. yielded positive reactions and 12 strains of non-Listeria were negative, resulting in a specificity of 100%. L. monocytogenes was artificially inoculated in raw pork macerated with listeria enrichment broth.
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