Contamination patterns of Listeria spp. in pork processing plants using random amplified polymorphic DNA
2005
Ha, S.Y. (Kangwon National University, Chunchon, Republic of Korea) | Choi, W.S. (Dongguk University, Gyeongju, Republic of Korea) | Bahk, G.J. (Korea Health Industry Development Institute, Seoul, Republic of Korea) | Hong, C.H. (Kangwon National University, Chunchon, Republic of Korea), E-mail: hongch@kangwon.ac.kr
This study was carried out to understand the contamination patterns of Listeria in pork processing plants. A total of 402 samples were collected from carcass, pork during processing, surfaces of equipment and environment, and 238 isolates of Listeria species were identified. L. innocua was found in 64.7% of the isolates, L. monocytogenes in 33.2%, and L. welshimeri in 2.1%. Random amplified polymorphic DNA (RAPD) analysis performed to investigate the origin and routes of Listeria contamination, showed 21 composite types of L. monocytogenes and 26 composite types of L. innocua. It was confirmed that Listeria contamination begins with contaminated incoming carcass and ever-present contaminants in the processing environments.
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