Sodium formate influence on pathogenic Escherichia coli detection time reduction
2005
Monkaityte, R.(Kaunas Univ. of Technology (Lithuania). Food Inst.) | Hechavarria Gola, T.L.(Universitat Rovira i Virgili, Tarragona (Spain). Dinamic Applied Biotechnology Innovation Centre)
By the conventional Escherichia coli H2 and CO2 gas production protocol the detection takes minimum 5 days. In this work reduced E. coli detection and identification time is presented improving conventional protocol steps: pre-enrichment, E. coli H2 and CO2 gas production and confirmation. Spectrophotometrical and viable counting results indicate that changing conventional E. coli pre-enrichment medium buffered peptone water (BPW)) to tryptic soy broth (TSB) pre-enrichment can be reduced from 18 h till 7 h. E. coli H2 and CO2 gas production in brilliant green bile broth (BGBL) medium can be obtained in 5 h at 41 deg C using sodium formate (HCOONa, 5 g/l) as an additive. Making E. coli confirmation by indole test directly in BGBl medium, as results showed it was possible, the time is saved. By this method E. coli detection time can be reduced from 5 days till 13 hours.
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