Isoenzymatic characterization of Eryngium eleganscham. ET schlechtpopulations of Tucuman province, Argentina
2001
Andrada, A.B. | Nasif, A. | Adrianapastoriza | Chaila, S.,Zootecnia UniversidadNacional de Tucuman (Argentina). Catedra de Genetica Catedra de Cana deAzucar
The genus Eryngium has about 230 species distributed in temperate andsubtropical zones of Europe and America. In South America the genus ispresented in Bolivia, Paraguay, Uruguay, Brazil and Argentina. In thenorthwest of Argentina and specially in Tucuman province, Eryngium elegansCham. et Schlecht is one of the aggressive weed that affects pasture areas aswell as economically important crops like sugarcane. E. elegans is a diploidspecies with regular meiosis and fertile seeds so that it can easily colonizedifferent ecological areas. The objective of this work was to identify thedifferent populations through the use of isoenzyme patterns of esterases andperoxidases and the application of cluster analysis. Discontinuous verticalpolyacrylamide gel electrophoresis was utilized and Jaccard SimilarityCoefficients was used for making the dendrogram. Profiles of peroxidases,showed a characteristic and distinct pattern in all samples, which explains inpart, the phenotypic variation found in the population. This was confirmed bythe low value of similarity index showed by the dendrogram. In esterases, acommon wide band, of different colour intensity was observed according to thelocality. Through the difference in the pattern of peroxidases it was thuspossible to determine intraspecific variations in the populations which can beused for characterization of samples.
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