Study on porcine circovirus infection of swine in Thailand
1999
Worawit Watchawalku | Teerapon Sirinarumitr | Sudarat Damrongwatanapokin | Tuangthong Patchimasiri(Department of Livestock Development, Bangkok (Thailand). National Institute of Animal Health)
Seven 4-10 week-old pigs with postweaning multisystemic wasting syndrome from Chonburi, Nakorn Pathom, Ratchaburi and Chachoengsao which exhibited intracytoplasmic inclusion bodies in lymphoid organs were selected for study of porcine circovirus infection. These pigs were infected by porcine circovirus (PCV) as confirmed by in situ hybridization, polymerase chain reaction (PCR) and DNA sequencing. Microscopic observation revealed pale firm reberry and non-collapsed lung (3/7), pale and enlarged lymph nodes (4/7), yellowish liver (4/7), pale kidneys (4/7), watery content in large intestine (2/7) and ascites (1/7). Histopathological study revealed intracytoplasmic inclusion bodies which were presented in tonsil (7/7), lymph nodes (5/7), spleen (3/7) and Peyer patches (2/7). Other histopathological findings included lymphoid depletion (7/7), interstitial pneumonia (5/7), purulent pneumonia (3/7), non-purulent encephalitis (3/7), degeneration of liver (6/7), degeneration and necrosis of pancreatic acinar cells (4/7) and granulomatous inflammation (2/7). Ultrastructurally, the inclusion bodies were composed of electron-dense paracrystalline arrays and whirl structure of small non-enveloped icosahedral viral particles that were approximately 17 nm in diameter. Therefore, the finding of intracytoplasmic inclusion bodies characterized by single globule and grape-like or botryoid clusters, basophilic and amphophilic staining by hematoxylin and eosin (H and E) can be used for diagnosis of porcine circovirus infection or postweaning multisystemic wasting syndrome (PMWS).
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