Controlling of malolactic fermentation and Lactobacillus brevis grow with immobilized onto membrane nisin
2007
Krastanov, A.
Nisin is an antimicrobial peptide proven to be an effective inhibitor of gram positive bacteria. The efficacy of this bacteriocin was examined after immobilization om polyethersulfonic membrane in a spiral UF-module. The 70-75% immobilization yield was achieved up to 10-15 minutes from the beginning of the process. The optimal concentration of nisin solution for immobilization is 0.25-0.30 mg/ml. Immobilized nisin demonstrate very good antimicrobial activity as the modules operate in continuous as in cycle mode. Total inhibition of L. brevis grow is observed when the cells is treated during lag-phase of cultivation, and no lactic acid was determined. Results indicate that immobilized on membrane nisin can be used to control malolactic fermentation.
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