QTL mapping for kernel yellow pigment content in common wheat

Chinese. 小麦面粉黄色度b. _凳欠从趁娣垩丈_闹匾_副辏_饕_胱蚜；粕_睾_坑泄亍＠__22对SSR引物、4对贮藏蛋白STS引物和10对AFLP引物组合，分析了中优9507×CA9632的71个DH系，构建了由173个位点组成的遗传连锁图，在小麦21个连锁群上覆盖2881cM。将该群体种植2年共计5个地点，测定籽粒黄色素和面粉黄色度b. _岛_俊２捎酶春锨_渥魍挤ǎCIM）进行了籽粒黄色素含量和面粉黄色度QTL分析。结果表明，面粉黄色度b. _档QTL位于染色体1DS、2DL、3A、4D、5D、6AL、6D和7AL上，其中7AL的QTL效应最大，贡献率为12．9％～37．6％；籽粒黄色素含量的QTL位于染色体2DL、3DL、4A、5A和7AL，其中7AL的QTL效应最大，贡献率为12．1％～33．9％。面粉黄色度b. _涤胱蚜；粕_睾_抗餐_QTL位于7AL，与Xgwm264b紧密连锁，遗传距离分别为0～3．9cM和0～0．9cM。

English. Flour yellowness b. _is a major criterion to estimate flour colour, which is basically determined by yellow pigment content in wheat grains. A doubled haploid （DH） population with 71 lines derived from a cross between Zhongyou 9507 with lower flour yellowness b. _and CA9632 with higher flour yellowness b. _, was sown for two years in 5 locations. A total of 173 loci including 122 SSR, 4 STS of storage protein genes and 10 AFLP markers were used to construct a linkage map, covering 2881 cM of whole genome at 21 linkage groups. With the method of composite interval mapping （CIM）, 8 putative major QTL of yellowness b. _were detected on chromosomes 1DS, 2DL, 3A, 4D, 5D, 6AL, 6D and 7AL （Table 2）, and 5 putative QTL of yellow pigment content were detected on chromosome 2DL, 3DL, 4A, 5A and 7AL （Table 3）, respectively. The QTL on 7AL was common for yellowness b. _and yellow pigment content, accounting for 12.9%-37.6% and 12.1%-33.9% of the phenotypic variance across different environments, respectively, which was closely linked to Xgwm264b. The genetic distance between Xgwm264b and the QTL of flour yellowness b. _and grain yellow pigment on chromosome 7AL were 0-3.9 cM and 0-0.9 cM, respectively. It can be used as a molecular marker in wheat breeding program.