Polyphenol content and antiradical activity in different apple varieties
2006
Lachman, J. | Sulc, M. | Sus, J. | Pavlikova, O.
Fifteen apple varieties were analysed for their total polyphenol content (TP) by two methods, by Folin-Ciocalteau reagent (FC) and by EBC method with carboxylmethylcellulose/sodium ethylendiamintetraacetate (CMC/EDTA). Their antiradical activity (ARA) was determined by the DPPH method using stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH·). TP and ARA were determined in freshly pressed apple juice and apples, and obtained results were statistically evaluated. The differences between varieties were significant. The correlation between the TP content determined by the FC method summarizing all free aromatic hydroxyls and the EBC method determining mainly ortho-aromatic hydroxyls was found with r=0.73. The highest differences among the analysed varieties were found for ARA values in both juice and apples and for TP content determined by FC in apples. High polyphenol contents were found in Jonagold, Jonalord, Melodie and Melrose varieties. On the contrary, low TP contents were estimated in Gloster and Rosana varieties. The highest ARA levels were found in Rajka, Bohemia and Melrose varieties, the lowest ones in Sampion and Topaz varieties.
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