Molecular differentiation of the four most commonly occurring Trichogramma (Hymenoptera: Trichogrammatidae) species in China
2007
Li, Z.X.,China Agricultural Univ., Beijing (China). Dept. of Entomology
Trichogramma dendrolimi, T. ostriniae, T. confusum and T. evanescens are the four most commonly occurring Trichogramma species with overlapping distribution in China. Different molecular markers were employed to molecularly characterize and differentiate these species, including direct amplification of the internally transcribed spacer 2 (ITS2) of ribosomal DNA by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and species-specific primers. The results showed that direct amplification of ITS2 could not clearly discriminate these species, but they could be differentiated using RFLP pattern obtained with endonucleases EcoRI and HindIII. The banding pattern produced by RAPD is irreproducible, so it is not a suitable way to identify Trichogramma species. Finally, the species-specific primers designed based on ITS2 sequences could unequivocally distinguish the four species. The species-specific primer-based protocol proved to be the most convenient and time saving method for the identification of Trichogramma species by creating a unique PCR product.
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