Molecular regulation of progesterone synthesis in the bovine corpus luteum
2007
Rekawiecki, R.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research | Kotwica, J.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research
The aims of this study were: (a) to determine whether progesterone affects the expression of the gene for its own receptor, thereby affecting its own synthesis; and (b) to determine whether oxytocin and noradrenaline affect the expression of the genes for the oxytocin receptor (OT-R), the progesterone receptor (P4-R), and the beta2 receptor (beta2-R), thereby regulating luteal steroidogenesis. Two populations of luteal cells were isolated from slaughtered cows and used in the present study: from 6th-10th (LC1) and 11th-16th (LC2) days of the estrous cycle. The cells were treated for six hours with one of the following hormones: luteinizing hormone (LH; 100 ng/mL); progesterone (P4; 10-*5 M); progesterone antagonist (10-*5 M); noradrenaline (10-*5 M); actinomycin D (500 ng/mL). The medium was then collected for the determination of progesterone concentration. With LH, the P4 concentration in the medium increased with both types of cells. None of the other treatments affected the progesterone concentration. The levels of expression of the genes for OT-R, P4-R and beta2-R were determined. Total RNA was extracted from cells, treated with DNase, and subjected to reverse transcription. Treatment with LH was the only treatment that increased the level of expression of the gene for P4-R in both LC1 and LC2. Both treatments with LH and P4 increased the level of expression of the gene for OT-R in LC1. The basal level of expression of the gene for OT-R was higher in LC1 than in LC2. This suggests that there is a positive feedback between progesterone and oxytocin, with both playing a role as a local factor that enhances the function of the corpus luteum.
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