Determination of glycarbylamide based on formation of nickel chelate
2006
Tajima, N.(Showa Univ., Tokyo (Japan). School of Pharmaceutical Sciences) | Mizuno, Y. | Hamamoto, K. | Arakawa, H. | Maeda, M.
A new and simple analytical method for glycarbylamide (GB) based on the formation of nickel chelate was developed. The proposed method is as follows: sample solution is mixed with 0.08 mmol/L nickel nitrate in 0.2 mol/L carbonate buffer (pH 9.0), and the absorbance is measured at 290 nm. Under the optimal conditions, GB could be determined in the concentration range from 0.13 microg/mL to 2.6 microg/mL (r=0.9999). Using this method for HPLC post column reaction, GB levels in chicken liver extracts could be determined. The recovery of GB was 79.4%(RSD=2.6%, n=3) and the quantitation limit was 30 ng/g. The apparent molar extinction coefficient (epsilon) of the GB-nickel complex was 8.5 x 10E3. The molar ratio of the complex is GB:nickel ion = 2:1.
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