Development of a Sensitive Bioassay Method for Quorum Sensing Inhibitor Screening Using a Recombinant Agrobacterium tumefaciens
2005
Kim, Y.H. (Pusan National University, Busan, Republic of Korea) | Kim, Y.H. (Pusan National University, Busan, Republic of Korea) | Kim, J.S. (Dongseo University, Busan, Republic of Korea), E-mail: [email protected] | Park, S.H. (Pusan National University, Busan, Republic of Korea)
Acylhomoserine lactones (AHLs) are known to be the triggering molecules in the quorum sensing mechanism of many gram-negative bacteria. In order to detect AHL inhibitors that are potential biofilm inhibitors, a convenient and sensitive bioassay was developed based on the β-galactosidase activity (β-GAL) of a recombinant Agrobacterium tumefaciens strain. A series of commercially available AHLs were tested for inducing β-GAL at varying concentrations in agar-plate and liquid cultures of the reporter strain.
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