detection of maize strips tenuivirus in Rottboellia cochinchinensis and its transmission to corn, Zea mays
2003
Navarro, J.T., Bajet, N.B.(Univ. Los Baños, College, Laguna (Philippines). Dept. of Plant Pathology)
Two types of foliar symptoms were observed on Rottboellia cochinchinensis in pots around the UPLB Biological Sciences building: Type A comprised of very small dots and thin pale yellow short lines along the veins and were more numerous and prominent on newly emerged leaf. The more commonly observed was Type B symptoms which consist of long and narrow chlorotic stripes wider at the base, taper and end toward the middle of the leaf and the developing younger leaves showed complete chlorosis. results of enzyme-linked immunosorbent assay(ELISA) showed that maize strips teneivirus (MS + pV), rice grassy stunt tenuivirus (RgSV), sugarcane/maize dwarf mosaic potyvirus (SC/MDMV), and maize mosaic rehabdovirus (MMV) were present in the symptomatic plants with MStpV the most commonly occurring virus with 77.0% of the samples infected by it. This virus was either alone (26.and%) or in combination (5 or 3 %) with \{c/MDMV, MMV or RGSV in the symptomatic R-co chinchinensis but the viruses were not detected in nonsymptomatic plants. At 15.8 kDa protein was observed with the MstpV-ELISA positive but not with MStpV-ELISA negative samples in SDS-RAGE gels. A signal corresponding to this protein appeared on the membrane probed with the MStpV centiserum in western blot indicating that R. cochinchinensis was infected with MStpV. The MStpV was transmitted from R. cochinchinensis to corn by the insect vector Peregrinus maidis that was infesting that plant but neither with the non-MStpV exposed vector nor from MStpV diseased R. cochinchinensis alone. Both R. exalta and the P. maidis serve as reservoir for MStpV. MStpV as a threat to corn production in the country is discussed.
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