Rapid detection of destructive fungal and bacterial pathogens of corn using PCR [polymerase chain reaction]
2004
Pascual, C.B.,Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding
Several PCR primers have been screened and tested to determine the primers that would be able to detect and identify different destructive pathogens of corn. ITS (internal transcribed spacer) primers of rDNA for Stenocarpella macrospora and Rhizoctonia solani, species-specific primer from pectate-lyases encoding gene for Pectobacterium chrysanthemi (syn. Erwinia chrysanthemi) and genus-specific primer for Peronosclerospora philippinensis were found to rapidly detect and accurately identify these microorganisms.
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