Effect of different routes of vaccination against newcastle disease on lymphoid organs of broilers
2002
Salam, R.
This project was designed to compare two routes (intraocular and drinking water) of vaccination against Newcastle disease in terms of protection against velogenic field isolate of NDV by noting immune response and morphological changes in Harderian gland, Bursa of Fabricius and thymus of broilers. This project would also help to evaluate role of Harderian gland to generate local and humoral immunity in response to eye drop and drinking water vaccination against NDV. For this purpose, a total of 160 day-old chicks were procured from local commercial hatchery. At day 7 birds were randomly divided into four groups i.e. A, B, C, D comprising 40 birds each. Group A and B were vaccinated through ocular and oral route respectively. While groups C and D) were kept is control. At day 21, groups A and B were revaccinated through the same routes. At day 28, groups A, B and C were further sub-divided into two subgroups each i.e. A1 and A2, B1 and B2, C1 and C2, respectively . Subgroups A1, B1 and C1 were challenged, ocularly while subgroups A2, B2 and C2 were challenged orally with velogenic field isolate of NDV. Ocular vaccination resulted in higher level of circulating antibodies and increased number of plasma cells in Harderian gland. It can be inferred that ocular vaccination stimulated Harderian gland to produce strong local protective immunity both in ocular and oral mucosa.
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