Protein changes associated with dedifferentiation and differentiation in vitro callus cultures of sugarcane (Saccharum spp. hybrid cv. CP-43/33)
2004
Aftab, F. (Punjab Univ., Lahore (Pakistan). Dept. of Botany) | Aftab, Z.H. | Iqbal, J.
In the present work, in vitro somatic embryo induction and stages in differentiation were studied from explants taken from immature leaves and pith of field grown sugarcane (Saccharum spp. hybrid cv. CP-43/33). Besides dedifferentiation and differentiation, quantitative changes in soluble protein content between non-embryogenic and embryogenic calluses were studied. For callus induction, MS medium supplemented with 3.0 in mgl-1 2, 4-D, 375 mgl-1 casein hydrolysate and 30 gl-1 sucrose gave the best results. Both embryogenic as well as non-embryogenic callus was induced from leaf explant. The soluble protein content showed a gradual increase from callus induction to fifth subculture in embryogenic and non-embryogenic calluses. An increase of soluble, protein content during embryo differentiation teas also observed during these studies.
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